Low Genetic Variability in the Hawaiian Monk Seal

The Hawaiian monk seal (   Monachus schauinslandi) is a critically endangered species that has failed to recover from human exploitation despite decades of protection and ongoing management efforts designed to increase population growth. The seals breed at five principal locations in the northwestern Hawaiian islands, and inter-island migration is limited. Genetic variation in this species is expected to be low due to a recent population bottleneck and probable inbreeding within small subpopulations. To test the hypothesis that small population size and strong site fidelity has led to low within-island genetic variability and significant between-island differentiation, we used two independent approaches to quantify genetic variation both within and among the principal subpopulations. Mitochondrial control region and tRNA gene sequences (359 base pairs) were obtained from 50 seals and revealed very low genetic diversity (0.6% variable sites), with no evidence of subpopulation differentiation. Multilocus DNA fingerprints from 22 individuals also indicated low genetic variation in at least some subpopulations (band-sharing values for "unrelated" seals from the same island ranged from 49 to 73%). This method also provided preliminary evidence of population subdivision (  F'_st estimates of 0.20 and 0.13 for two adjacent island pairs). Translocations of seals among islands may therefore have the potential to relieve local inbreeding and possibly to reduce the total amount of variation preserved in the population. Genetic variation is only one of many factors that determine the ability of an endangered species to recover. Maintenance of existing genetic diversity, however, remains an important priority for conservation programs because of the possibility of increased disease resistance in more variable populations and the chance that inbreeding depression may only be manifest under adverse environmental conditions.     

Variation in secondary metabolite and aragonite concentrations in the tropical green seaweed Neomeris annulata: effects on herbivory by fishes

The tropical alga Neomeris annulata (Dickie) (Dasycladaceae: Chlorophyta) produces brominated sesquiterpenes and deposits aragonite throughout the thallus. This study, conducted throughout 1990–1991, showed that the fleshy, apical portions of the thalli (tips) were high in secondary metabolite concentrations (1.5%, mean combined secondary metabolites based on dry mass) and relatively low in calcium carbonate (aragonite form) (65.2% ash). The basal portions were lower in combined secondary metabolites (0.2% dry mass), and higher in aragonite (90.0% ash). The crude organic extract of the alga deterred fish feeding in the field at concentrations of 5, 10 and 15% dry mass, but not at a lower concentration of 1.5%. Natural concentrations of crude organic extract ranged from 1.5 to 15.3% in whole individuals and averaged 5.1% based on dry mass. Two brominated sesquiterpenes were isolated as major metabolites from the crude extract, but only one deterred feeding at natural concentrations. Ash concentrations in N. annulata were 60% dry mass in both the tips and bases. Aragonite strongly deterred feeding at concentrations of 65 and 90% dry mass. When a naturally occurring combination of organic extract and aragonite in the tips (10% crude extract and 65% aragonite) was compared with that of the bases (0.8% crude extract and 90% aragonite), no significant difference in grazing was observed. Combinations of secondary metabolites and aragonite were also tested against one or the other single defense. The combination of defenses proved a more effective deterrent than either secondary metabolites or aragonite alone.     

Continuous determination of fine particulate matter mass in the Salt Lake City Environmental Monitoring project: a comparison of real-time and conventional TEOM monitor results

Fine particulate matter (PM2.5) mass was determined on a continuous basis at the Salt Lake City Environmental Protection Agency Environmental Monitoring for Public Awareness and Community Tracking monitoring site in Salt Lake City, UT, using three different monitoring techniques. Hourly averaged PM2.5 mass data were collected during two sampling periods (summer 2000 and winter 2002) using a real-time total ambient mass sampler (RAMS), sample equilibration system (SES)-tapered element oscillating microbalance (TEOM), and conventional TEOM monitor. This paper compares the results obtained from the various monitoring systems, which differ in their treatment of semivolatile material (SVM; particle-bound water, semivolatile ammonium nitrate, and semivolatile organic compounds). PM2.5 mass results obtained by the RAMS were consistently higher than those obtained by the SES-TEOM and conventional TEOM monitors because of the RAMS ability to measure semivolatile ammonium nitrate and semivolatile organic material but not particle-bound water. The SES-TEOM monitoring system was able to account for an average of 28% of the SVM, whereas the conventional TEOM monitor loses essentially all of the SVM from the single filter during sampling. Occasional mass readings by the various TEOM monitors that are higher than RAMS results may reflect particle-bound water, which, under some conditions, is measured by the TEOM but not the RAMS.     

The geological setting of lode-gold deposits in the central southern Urals: a review

The late-Paleozoic Uralides represent one of the largest lode-gold metallogenic provinces in the world. In the southern Urals, gold distribution is heterogeneous and is confined mainly to two tectonostratigraphic zones, namely the Main Uralian fault and the East Uralian zone. The important lode-gold districts within and in the immediate hangingwall of the first-order crustal suture of the Main Uralian fault are characterized by a complex tectonic history of earlier compressional tectonics involving thrusting, folding and reverse faulting and later transcurrent shearing. Gold mineralization is hosted by second- and third-order brittle to brittle–ductile strike-slip faults that developed late during the kinematic history of the Main Uralian fault. Strike-slip reactivation of earlier compressional structures was related to the late-stage docking of the passive margin of the East European platform with island-arc complexes of the southern Urals, an event that is tentatively related to changes in plate motion during the final stages of terrane accretion during the upper Permian and lower Triassic. Gold mineralization was controlled by the permeability characteristics of the hydrothermal conduits, as well as by competence contrasts and geochemistry of the mainly volcanic host rocks. Mineralization occurred at relatively shallow crustal levels (2–6 km) and largely post dates peak-metamorphism of the host rocks. The large and very large (up to 300 to Au) gold deposits of the East Uralian zone are hosted by upper-Paleozoic granitoid massifs. Gold mineralization is temporally associated with the main phase of regional-scale compressional tectonics and granite plutonism during the upper Carboniferous and lower Permian. Controlling structures have a dominantly east–west strike and occur as hybrid shear-tensional vein systems in competent granitoids subjected to east/west-directed regional shortening. Deformation textures and alteration mineral assemblages indicate lower-amphibolite-facies conditions of mineralization close to peak metamorphic conditions that are associated with the mid-Permian regional metamorphism and tectonism. Gold deposits in the southern Urals are, therefore, polygenetic and are temporally and genetically distinct in each of the two major mineralized tectonostratigraphic zones of this well-preserved collisional orogenic belt. The different timing of ore fluid generation and fluid discharge is interpreted to be the result of the different tectonic, metamorphic and magmatic evolution of terranes in the southern Urals.